Isabelle MILA
Assistant Ingénieur INRA
+33 (0)5 34323868
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Responsable du système expérimental d’étude de la régulation transcriptionnelle appelé « single cell»
Je suis en charge d'un système qui permet une approche fonctionnelle des interactions protéines/ADN et protéines/protéines impliquées dans la régulation de l’expression de gènes cibles.
Carrière
2002-aujourd’hui | Assistant Ingénieur, Laboratoire de Génomique et Biotechnologie des fruits, UMR 990 INPT/ENSAT-INRA, INRA Auzeville |
2002-1998 | Assistant Ingénieur, Laboratoire de chimie Biologique, équipe Lignines, INAPG, INRA Grignon |
1992-1998 | Technicienne de Recherche, Laboratoire de chimie Biologique, équipe Tannins, INAPG, INRA Grignon |
1998 | Diplôme d’Ingénieur en Biochimie des IAA, CNAM, Paris |
1991 |
Diplôme de Licence Professionnelle en Biotechnologies Végétales, ENFA-UPS Toulouse |
Compétences:
Biologie Moléculaire, Culture in vitro,Transformation transitoire, Microscopie confocale à balayage,Cytométrie en flux
Activités transversales:
- Hygiène et sécurité : Agent chargé de prévention de l'unité, sauveteur secouriste du travail, représentante du personnel au CHSCT et membre du groupe Risques Psycho-Sociaux du centre INRA de Toulouse
- Démarche Qualité : Responsable métrologie
Publications
2019
- Shin JH, Mila I, Liu M, Rodrigues MA, Vernoux T, Pirrello J, Bouzayen M. The RIN-regulated Small Auxin-Up RNA SAUR69 is involved in the unripe-to-ripe phase transition of tomato fruit via enhancement of the sensitivity to ethylene. New Phytol. 2019 Apr;222(2):820-836. doi: 10.1111/nph.15618.
2018
- Klay I, Gouia S, Liu M, Mila I, Khoudi H, Bernadac A, Bouzayen M, Pirrello J. Ethylene Response Factors (ERF) are differentially regulated by different abiotic stress types in tomato plants. Plant Sci. 2018 Sep;274:137-145. doi: 10.1016/j.plantsci.2018.05.023.
- Djemal R, Mila I, Bouzayen M, Pirrello J, Khoudi H. Molecular cloning and characterization of novel WIN1/SHN1 ethylene responsive transcription factor HvSHN1 in barley (Hordeum vulgare L.). J Plant Physiol. 2018 Sep;228:39-46. doi: 10.1016/j.jplph.2018.04.019.
- Liu M, Chen Y, Chen Y, Shin JH, Mila I, Audran C, Zouine M, Pirrello J, Bouzayen M. The tomato Ethylene Response Factor Sl-ERF.B3 integrates ethylene and auxin signaling via direct regulation of Sl-Aux/IAA27.New Phytol. 2018 Jul;219(2):631-640. doi: 10.1111/nph.15165.2017.
- Bouzroud S, Gouiaa S, Hu N, Bernadac A, Mila I, Bendaou N, Smouni A, Bouzayen M, Zouine M. Auxin Response Factors (ARFs) are potential mediators of auxin action in tomato response to biotic and abiotic stress (Solanum lycopersicum). PLoS One. 2018 Feb 28;13(2):e0193517. doi: 10.1371/journal.pone.0193517.
2017
- Huang B, Routaboul JM, Liu M, Deng W, Maza E, Mila I, Hu G, Zouine M, Frasse P, Vrebalov JT, Giovannoni J, Li Z, van der Rest B and Bouzayen M. Overexpression of class D MADS-box Sl-AGL11 impacts fleshy tissue differentiation and structure in tomato fruit. J Exp Bot. 2017 Sep 14. doi: 10.1093/jxb/erx303.
2015
- Hao Y., Hu G., Breitel D., Liu M., Mila I., Frasse P., Aharoni A., Bouzayen M. and Zouine M. (2015) Auxin Response Factor SlARF2 Is an Essential Component of the Regulatory Mechanism Controlling Fruit Ripening in Tomato. PLoS Genet Dec 30;11(12):e1005649. doi: 10.1371/journal.pgen.1005649. eCollection 2015.
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Mazzucato, A., Cellini, F., Bouzayen, M., Zouine, M., Mila, I., Minoia, S., Petrozza, A., Picarella, M.E., Ruiu, F. and Carriero, F. (2015) A TILLING allele of the tomato Aux/IAA9 gene offers new insights into fruit set mechanisms and perspectives for breeding seedless tomatoes. Molecular Breeding, 35, 1-15.
2014
- Zouine, M., Fu, Y., Chateigner-Boutin, A.-L., Mila, I., Frasse, P., Wang, H., Audran, C., Roustan, J.-P. and Bouzayen, M.(2014) Characterization of the tomato ARF gene family uncovers a multi-levels post-transcriptional regulation including alternative splicing.
2013
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M. Sagar, C. Chervin, I. Mila, M. Benichou,Y. Gibon, B. Biais, M. Bouzayen, M. Zouine, 2013. Sl-ARF4, an Auxin Response Factor involved in the control of sugar metabolism during tomato fruit development. Plant Physiol
2012
- Pirrello J, Prasad N, Zhang W, Chen K, Mila I, Zouine M, Latche A, Pech JC, Ohme-Takagi M, Regad F, Bouzayen M (2012) Functional analysis and binding affinity of tomato ethylene response factors provide insight on the molecular bases of plant differential responses to ethylene. BMC Plant Biol 12: 190
- Bassa C., Mila I., Bouzayen M., Audran-Delalande C. (2012). Phenotypes associated with down-regulation of Sl-IAA27 support functional diversity among Aux/IAA family members in the tomato. Plant and Cell Physiology 53(9): 1583-1595
- Audran-Delalande C., Bassa C., Mila I., Regad F., Zouine M. and Bouzayen M. (2012). Genome-wide identification, functional analysis and expression profiling of the Aux/IAA gene family in tomato. Plant and Cell Physiology 53(4): 659-672.
- Deng W., Yang Y., Ren Z, Audran-Delalande C., Mila I., Wang X., Song H., Hu Y., Bouzayen M. and Li Z.G. (2012). The tomato SlIAA15 is involved in trichome formation and axillary shoot development . New Phytologist 194: 379-390.
2010
- El-Sharkawy I, Mila I, Bouzayen M, Jayasankar S. (2010). Regulation of two germin-like protein genes during plum fruit development. J Exp Bot.(6):1761-70.
2009
- El-Sharkawy I, Sherif S, Mila I, Bouzayen M, Jayasankar S. (2009) Molecular characterization of seven genes encoding ethylene-responsive transcriptional factors during plum fruit development and ripening. J Exp Bot.;60(3):907-22.
- Chaabouni S.,Jones B., Delalande C.,Wang H., Li Z.G., Mila I., Frasse P., Latche A., Pech J.C. and Bouzayen M. (2009). Sl-IAA3, atomato Aux/IAA at the crossroads of auxin and ethylene signalling involved in differential growth.
2008 -2006
- Hommel M, Khalil-Ahmad Q, Jaimes-Miranda F, Mila I, Pouzet C, Latché A, Pech JC, Bouzayen M, Regad F, (2008). Over-expression of a chimeric gene of the transcriptional co-activator MBF1 fused to the EAR repressor motif causes developmental alteration in Arabidopsis and tomato. Plant Science, 175(1-2): 168-177.
- Auldridge ME, Block A, Vogel JT, Dabney-Smith C, Mila I, Bouzayen M, Magallanes-Lundback M., DellaPenna D, McCarty DR, Klee HJ, (2006). Characterization of three members of the Arabidopsis carotenoid cleavage dioxygenase family demonstrates the divergent roles of this multifunctional enzyme family. Plant Journal; 45(6):982-933-B.C.